The scope of this KPI includes company-level activities for manufacturers that sell beauty and personal care products in the United States. Except where noted, company-level activities in other regions are not to be considered.

Manufacturers that are performing animal testing but not performing any of the activities associated with B - E should select response option A.

  • For A, a manufacturer, or their raw material suppliers, may perform animal testing or a manufacturer may be unable to verify that their suppliers do not perform animal testing on the raw materials they produce.
  • For B, animal testing can be performed using validated alternative methods which refine or replace the use of animals.  In the US, alternative methods are validated by ICVAAM and accepted by regulatory agencies.  The complete list of acceptable alternative methods for the US can be found using the National Toxicology Program (NTP) link in the Resources section.
  • For C, animal testing cannot be performed to obtain safety data for safety justification of products sold in the United States.  Any data obtained from mandatory animal testing per regulatory requirements in regions outside of the United States cannot be used to substantiate ingredient or formulation safety for products sold in the United States.
  • For D and E, contributions to research initiatives and efforts to reduce regulatory requirements for animal testing include those outside of the United States.
  • For D, major research initiatives are government, university, or privately based programs that are dedicated to the replacement or refinement of animal testing by advancing non-animal alternative testing methods through effective development, validation, use, or communication.  Examples of major research initiatives include, but are not limited to, those listed in the Resources section.


National Toxicology Program (NTP) Alternative Methods Accepted by US Agencies:  This website lists the testing methodologies that have been accepted or endorsed by US and EU regulation.

Japanese Center for the Validation of Alternative Methods (JaCVAM):  JaCVAM is an institute that is dedicated to the promotion of the reduction, refinement, and replacement of animal testing used to justify chemical safety in Japan. This mission is achieved in part through international collaboration.

The European Unioin Referencen Laboratory for Alternatives to Animal Testing (EURL ECVAM):  EURL ECVAM is dedicated to the advancement of animal testing alternatives by promoting non-animal alternatives through scientific research, validation, and independent evaluation. ECVAM’s ultimate goal is enhanced safety at multiple life cycle stages with decreased reliance on animal testing.  

Center for Alternatives to Animal Testing (CAAT):  The Johns Hopkins Center for Alternatives to Animal Testing provides a valuable resource for organizations seeking information about alternatives to animal testing. CAAT promotes the science and support of animal alternatives, their creation, development, and validation.

Centre for Documentation and Evaluation of Alternatives to Animal Experiments (AnimAlt-ZEBET):  The ZEBET database is part of the German Centre for the Protection of Laboratory Animals and is a valuable resource for industry, universities, and the public to access and understand information regarding animal testing alternatives.

The Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM):  ICCVAM is an interagency committee composed of representatives from various U.S. federal regulatory and research agencies with the goal of facilitating the development and ultimate regulatory acceptance of test methods that reduce, refine, or replace animal testing.


Validated alternative methods: Testing methodologies that reduce, refine, or replace the use of animals and have been validated by ICVAAM in the United States and accepted by regulatory agencies for data collection.